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8883澳门新莆京老版本--同裂酶对照表

来源于不同物种但能识别相同DNA序列的限制性内切酶，被称为同裂酶（isoschizomer）。同裂酶切割位点可以相同也可以不同；识别序列和切割位点都相同的叫同序同切酶，识别序列相同但切割位点不同的叫同序异切酶。

因为各家公司产品开发策略的不同，有多种同序同切酶同时在市场上销售。为方便客户选择，8883澳门新莆京老版本给出同裂酶索引表格，方便大家选择合适的内切酶。

Catalog #	Product	Isoschizomers	Cut site	Optimal reaction temperature	Thermal inactivation	Methylation Effects
EG15601S	LightNing™ ApaLI	Alw44I, VneI	G/TGCAC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect.
EG15508S	LightNing™ AscI	PalAI，SgsI	GG/CGCGCC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15509S	LightNing™ AvrII	AspA2I, XmaJI, BlnI	C/CTAGG	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15510S	LightNing™ BamHI		G/GATCC	37℃	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15513S	LightNing™ BclI	BsiQI, FbaIC6:C1322I	T/GATCA	37℃	80℃, 20 min	Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
EG15591S	LightNing™ BglII		A/GATCT	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped.
EG15518S	LightNing™ BsaI	Eco31I, Bso31I, BspTNI	GGTCTC(1/5)	37℃	80℃, 20 min	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect.
EG15607S	LightNing™ BstBI	AsuII, Bpu14I, BspT104I, Bsp119I, Csp45I, NspV, SfuI	TT/CGAA	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoBI: no effect.
EG15528S	LightNing™ BstEII	Eco91I, BstPI, EcoO65I, PspEI	G/GTNACC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect.
EG15532S	LightNing™ ClaI	BspDI，BanIII，Bsa29I，BseCI，BshVI，BsiXI，Bsp106I，BspXI，Bsu15I，BsuTUI，ZhoI	AT/CGAT	37℃	80℃, 20 min	Dam: blocked if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect.
EG15585S	LightNing™ DpnI	MalI	GA^M6/TC	37℃	80℃, 20 min	Dam: does not cut dam- DNA. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped.
EG15533S	LightNing™ DpnII	BfuCI，MboI，Sau3AI，BscFI，Bsp143I, BssMI，BstENII，BstMBI，Kzo9I，NdeII	/GATC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect.
EG15535S	LightNing™ EagI	BseX3I, BstZI, EclXI, Eco52I, XmaIII	C/GGCCG	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15536S	LightNing™ EcoRI	FunII	G/AATTC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
EG15537S	LightNing™ EcoRV	Acc16I, AviII, FspI	GAT/ATC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
EG21503S	LightNing™ Esp3I (BsmBI)	BsmBI, BstGZ53I, Esp16I, Esp23I	CGTCTC (1/5)	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect.
EG15586S	LightNing™ FspI	Acc16I, Avill, MstI, NsbI	TGC/GCA	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15539S	LightNing™ HindIII		A/AGCTT	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped.
EG15593S	LightNing™ HinfI		G/ANTC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: blocked if overlapped.
EG15541S	LightNing™ HpaI	BstHPI, KspAI	GTT/AAC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: no effect.
EG15543S	LightNing™ KasI	BbeI, DinI, EgeI, EheI, Mly113I, NarI, SfoI	G/GCGCC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15544S	LightNing™ KpnI	Asp718I	GGTAC/C	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15547S	LightNing™ MluI		A/CGCGT	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15548S	LightNing™ MnlI		CCTC(7/6)	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
EG15550S	LightNing™ NcoI	Bsp19I	C/CATGG	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15551S	LightNing™ NdeI	FauNDI	CA/TATG	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15552S	LightNing™ NheI	AsuNHI	G/CTAGC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
EG15553S	LightNing™ NotI	CciNI	GC/GGCCGC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15602S	LightNing™ NruI	Bsp68I，BtuMI，RruI	TCG/CGA	37℃	No (column purification)	Dam: blocked. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15606S	LightNing™ NsiI	EcoT22I, Mph1103I, Zsp2I	ATGCA/T	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
EG15603S	LightNing™ PacI		TTAAT/TAA	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect.
EG15561S	LightNing™ PstI	BspMAI	CTGCA/G	37℃	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15604S	LightNing™ PvuII		CAG/CTG	37℃	No (column purification)	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect.
EG15565S	LightNing™ SacI	Eco53kI, EcoICRI, Psp124BI, SstI	GAGCT/C	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15605S	LightNing™ SacII	Cfr42I，KspI，Sfr303I，SgrBI	CCGC/GG	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15566S	LightNing™ SalI		G/TCGAC	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15568S	LightNing™ SbfI	SdaI, Sse8387I	CCTGCA/GG	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15570S	LightNing™ SfiI		GGCCNNNN/NGGCC	50℃	No (column purification)	Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect.
EG15572S	LightNing™ SmaI	TspMI, XmaCI, XmaI	CCC/GGG	25℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect.
EG15574S	LightNing™ SpeI	AhlI, BcuI	A/CTAGT	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect.
EG15575S	LightNing™ SphI	BbuI, PaeI, SpaHI	GCATG/C	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped.
EG15576S	LightNing™ SspI		AAT/ATT	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15577S	LightNing™ StuI	AatI, Eco147I, PceI, SseBI	AGG/CCT	37℃	80℃, 20 min	Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect.
EG15580S	LightNing™ TaqI	TthHB8I	T/CGA	65℃	No (column purification)	Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15581S	LightNing™ XbaI		T/CTAGA	37℃	80℃, 20 min	Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.
EG15583S	LightNing™ XhoI	PaeR7I，TliI，BssHI，Sfr274I，SlaI，StrI	C/TCGAG	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect.
EG21501S	Sgel		m⁵CNNG(9/13)	37℃	80℃, 20 min	Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect.

8883澳门新莆京老版本-同裂酶对照表

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